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文献和实验A c-Kit (2B8) FITC-IgG2b, 01904D FITC-IgG2b Isotype control, 11184C Fc RII block (clone 2.4G2), 01241D Media: Heat inactivated FBS (56°C x 30 min) PBS + 2% heat-inactivated
antibody at 5 µg/ml for 45 min at 4 °C. Mouse anti-human NFB p65 monoclonal antibody sc-8008 (Santa Cruz, CA, USA) recognizes epitopes mapping the C amino-acid terminus of human origin NFB p65. After two washes in PBS–FCS, nuclei were incubated for 45 min
Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
PE‐labeled anti‐human HLA‐A,B,C antibody (4 µl/200 µl cells; BD Biosciences, cat. no. 555553) Mouse IgG1 FITC antibody (4 µl/200 µl cells; BD Biosciences, cat. no. 555748
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