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文献和实验Transplantation Models to Characterize the Mechanisms of Stem Cell–Induced Islet Regeneration
Monoclonal anti‐insulin antibody (Sigma, cat. no. I2018) Horse anti‐mouse IgG antibody, peroxidase‐labeled (Vector Labs, cat. no. PI‐2007
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
and has high inherent activity but is inhibited by activation of Wnt signalling oractivation of growth factor receptor tyrosine kinases (e.g. insulin, nerve growth factor [NGF], plateletderivedgrowth factor [PDGF], etc.). The degree of inhibition of GSK
polyclonal antisera and for mouse monoclonal antibodies from the IgG2a, IgG2b, and IgG3 subclasses. Protein G Sepharose is preferred for mouse IgG1 monoclonal antibodies and for polyclonal antisera from mouse, rat, sheep, and goat. Proteinase K
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