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文献和实验at least twice. If convinced that the subclone is monoclonal, checking the isotype is possible . If not convinced that the hybrydoma is monoclonal, reclone a second time. Cloning of Hybridoma Make an accurate cell count of cells
The production of monoclonal antibody by hybridoma
We outline a simple and contemporary protocol for the development of monoclonal antibodies using hybridoma fusion in immune mice (1). While the basic style of this fusion is similar to others (2,3), this protocol
Expanding Hybridoma（杂交瘤细胞） Clones
that the subclone is monoclonal, checking the isotype is possible . If not convinced that the hybrydoma is monoclonal, reclone a second time. Cloning of Hybridoma Make an accurate cell count of cells in log phase (3 E5 - 1E6 cells / ml).
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