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文献和实验from a 1 mg/ml stock in water. Store frozen in aliquots. Protein G Plus-Agarose From Oncogene Science (Cat # IP04). This product, which is ready to use, is 30% (v/v) agarose by volume and contains 20 mg IgG binding capacity/ml packed beads. Procedure
ANALYSIS OF PROTEINS BY IMMUNOPRECIPITATION
From Oncogene Science (Cat # IP04). This product, which is ready to use, is 30% (v/v) agarose by volume and contains 20 mg IgG binding capacity/ml packed beads. Procedure 1) Prepare samples as for Procedure I. For each sample tested, prepare 2 aliquants
Protocol for intracytoplasmic staining of cytokines for FACS analysis
Description Protocol for intracytoplasmic staining of cytokines for FACS analysis Procedure 1) Prepare spleen, lymph node or T cell clone cells as single cell suspension, using erythrocyte lysis (water) or Lymphopaque as appropriate
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