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文献和实验Indirect Immunostaining on Mouse Embryonic Heart for the Detection of Proliferated Cardiomyocyte
3 a marker for labeling proliferating cells. In this manuscript, we described two immunostaining methods (immunofluorescence and immunohistochemistry) for detecting the pH3-positive cardiomyocytes in formalin-fixed, paraffin-embedded heart tissues.
Isotype-specific immunostaining with mouse primary antibodies on mouse tissue
Staining mouse tissue with anti-mouse Ig (H&L chain) secondary reagents inevitabily causes staining of interstitial immunoglobulins, B cells, plasma cells and macrophages with Ig bound to Fc
Immunostaining with Anti-Digoxigenin Alkaline Phosphatase
Atlternate BCIP/NBT (Roche, #11697471001, 20 Tabs, makes 10 mL/Tab, $67) Blocking Reagent (Roche #11096176001, 50 g, $104, or 10x Block Buffer #11585762001, 1 set, $147) Alternate blocking reagents: Nonfat dry milk, mouse serum, goat serum. Tris
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