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文献和实验Use of Monoclonal Antibodies in Immunohistochemistry
with its expanding use of routinely processed, paraffin-embedded tissue sections, an achievement made possible by a series of technical accomplishments that include increased sensitivity of detection, monoclonal antibody (MAb) technology, and antigen retrieval
Monoclonal Antibody Production
(note the removal of the HT)in a T25 flask (one for each positive clone)for later use (7) When the cells are ready for expansion out of the 24-well plate,resuspend them and pipet to the T25 flasks with feeder cells.Again leave a little bit in the 24
Polyclonal and Monoclonal Antibodies
the serum. 4) Serum can be stored at -20 C after adding Glycerol to 50%. If monoclonal antibodies are desired, once you have a "good" polyclonal and a reliable screening method proceed to the next step. Immunization of Rabbits. Rabbits
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