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文献和实验Mapping Protein Distributions on Polytene Chromosomes by Immunostaining
serum, obtained from the same species as the secondary antibody. Depending on the method employed, use anti-rabbit IgG (Fc) horseradish peroxidase (HRP) conjugate (1:100 dilution), a biotin-conjugated secondary antibody, or a fluorescence-conjugated
All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies
with formalin-fixed paraffin embedded tissues, but with some frozen tissues, you may need to use 3% H2O2 to obtain optimal results (better morphology or lower background). To use 3% H2O2 for blocking endogenous peroxidase activity, incubate the slides in 3% H2
Reprogramming Fibroblasts with the CytoTune-iPS Reprogramming Kit
after transduction (Step 6), fibroblast cells are ready to be harvested and plated on MEF culture dishes. Remove the medium from the fibroblasts, and wash cells once with DPBS. b. To remove the cells from the 6-well plate, use 0.5 mL of TrypLE™ Select
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