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文献和实验Preparation of Polyclonal Antibodies to Retinoid Receptors
with antibodies to retinoic acid receptor a (RARα) enabled visualization of the promyelocyte-retinoic acid receptor (PML-RAR) fusion oncoprotein in cells from acute promyelocytrcleukemia patients (5 , 6 ) This chapter describes basic protocols for polyclonal
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
Polyclonal Antibody Production
to the pre-bleed, but get at least 20 mL per rabbit, with 40 mL being the upper limit for an adult rabbit (any more and they get anemic). Monitoring of Titer The quality of the antibodies in serum (humoral immune response) of the bleeds
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