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文献和实验Generation of Recombinant Adeno-Associated Virus
as a potential vector for gene therapy. For example, AAV is not pathogenic, approx 80% of adults in the United States are seropositive, but in no case has the virus been implicated as the etiological agent for a human disease. AAV is a defective parvovirus
genes to AAV2 is listed to the left of the ORF. Within the rep gene, two promoters, located at map units 5 and 19, direct the expression of four proteins, Rep78 and Rep68, and Rep52 and Rep40, respectively. Each promoter expresses one spliced (Rep68
表达的4种不同病毒样颗粒(VLPs)，再与藻红蛋白(PE)标记的、型特异性的单克隆抗体(Mabs)充分反应，使VLPs与MAb—PE充分结合，而样本中的型特异性的HPV—VLP抗体与VLPs亲和力比MAb—PE更高，从而抑制VLPs与MAb—PE的结合。因而报告分子MAb—PE的荧光信号强度与血清中的HPV—VLP抗体滴度成反比。可通过检测报告分子MAb—PE的平均荧光强度(MFI)进而对HPV6、l1、16、18型特异性的中性抗体进行定量检测。Biagini等运用该技术分析了人炭疽毒素IgG
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