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文献和实验The Use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in Salmonella
flagellin segments containing the export signal combined with a hexahistidine tag can be attached to heterologous proteins (preferentially in the size range of 9–40 kDa) facilitating their secreted expression and easy purification from the medium. Certain
Assaying Nuclear Messenger RNA Export in Human Cells
This chapter describes a simple method for the analysis of nuclear messenger RNA (mRNA) export in human cells in culture. The assay described relies on the observation that mRNA molecules containing an intron are generally
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells
Noninvasive Human Nuclear Transfer with Embryonic Stem Cells Sohyun L. McElroy1 and Renee A. Reijo Pera Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine
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