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文献和实验Functional Reconstitution of the Human Epithelial Na+ Channel in a Mammalian Expression System
recombinant ion channels in a mammalian expression system quantifying channel activity with patch clamp electrophysiology. Here, we describe investigation of the human epithelial Na+ channel heterologously expressed in Chinese hamster ovary cells.
Measurement of Chloride Movement in Neuronal Preparations
Inglefield, J.R. and Schwartz‐Bloom, R.D. 1997. Confocal imaging of intracellular chloride in living brain slices: Measurement of GABAA receptor activity. J. Neurosci. Methods. 75:127‐135.
Medium-Throughput Production of Recombinant Human Proteins: Protein Production in E. coli
In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E . coli system for test expressing intracellular
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