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文献和实验the concentration of unbound FA is a method using the fluorescent-(acrylodan) labeled intestinal FA-binding protein (ADIFAB). Under appropriate conditions, ADIFAB fluorescence measures unbound FA, regardless of any third phase such as albumin, FA-binding proteins
Methods for Producing Recombinant Human Cellular Retinaldehyde-Binding Protein
The cellular retinaldehyde-binding protein (CRALBP) is expressed at high levels in vertebrate visual trssue, where it may serve to modulate the interaction of 11-cis -retinol with visual-cycle enzymes in the retinal pigment epithelium (RPE
Fluorescence Assays for Measuring Fatty Acid Binding and Transport Through Membranes
The authors’ laboratory has applied a series of different fluorescence assays for monitoring the binding and transport of fatty acids (FA) in model and biological membranes. The authors recently expanded their fluorescent assays
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