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文献和实验High Throughput Production of Recombinant Human Proteins for Crystallography
This chapter presents in detail the process used in high throughput bacterial production of recombinant human proteins for crystal structure determination. The core principles are: (1 ) Generating at least 10 truncated constructs
Glucuronidation of Fatty Acids and Prostaglandins by Human UDP-Glucuronosyltransferases
glucuronidation from both human liver micro-somes and human recombinant UDP-glucuronosyltransferase 2B7 (UGT2B7) are presented. Several unique methods for synthesis of FAs that are not commercially available are also described. Moreover, comprehensive methods
Human Chromosome Analysis and Sorting
of small but useful quantities of individual chromosomes, for example, 2�106 average sized human chromosomes are equivalent to 500 ng of DNA. Using recombinant DNA techniques, this material can be used to generate a large number of DNA probes to produce
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