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文献和实验Controlled Expression of Recombinant Genes and Preparation of Cell-Free Extracts in Yeast
Biochemistry is an important experimental tool in the study of protein functions. Biochemical studies frequently involve overexpression of a cloned gene and purification of the recombinant protein. The yeast Saccharomyces cerevisiae provides
Generation of Spatio‐Temporally Controlled Targeted Somatic Mutations in the Mouse
of ligand?activated site?specific Cre recombinases has led to the development of cell type?specific temporally controlled targeted somatic mutagenesis in the mouse. We illustrate this technique using K14?Cre?ERT2 transgenic mice that express the tamoxifen
Developing Genetically Engineered Mouse Models to Study Tumor Suppression
. This review will describe the technical aspects of generating these mice and provide examples of the outcomes obtained from alterations of different tumor suppressors. Curr. Protoc. Mouse Biol. 2:9?24 © 2012 by John Wiley & Sons, Inc.
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