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文献和实验Antisense oligonucleotides have been used to study the structure and function of small nuclear ribonucleoprotein (snRNP) complexes and were adapted and modified for the purification of a variety of RNPs. We describe methods for recombinant
Yeast Nuclear Extract (Small Scale)
13 K rpm for 30 min.in SS34 rotor.Remove supernatant by dumping and discard.Drain pellets. Resuspend crude nuclear pellets with a small spatula in 10 ml Buffer B and transfer to 50 ml screwcap tubes.The prep can be stopped at this point.Quick freeze
Construction of Recombinant Adenoviruses that Produce Infectious Hepatitis B Virus
by the lack of infection systems that would allow testing. Only primary human or humanoid hepatocytes are fully permissive for HBV infection. Consequently, no permissive cell line and no convenient small animal model are available to study HBV infection
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