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文献和实验Transvascular delivery of small interfering RNA to the centr
of fully or partially sequence-complementary target mRNAs. In order to identify miRNAs and to assess their expression patterns, we sequenced over 250 small RNA libraries from 26 different organ systems and cell types of human and rodents that were enriched
Reconstitution of Nuclear Import in Permeabilized Cells
factors. The latter include receptors (karyopherins) that recognize the cargoes and carry them across the nuclear pore complex (NPC), and the small GTPase Ran, which modulates karyopherin–cargo binding. Nuclear import can be studied in vitro using
MicroRNA Biogenesis: Isolation and Characterization of the Microprocessor Complex
syndrome critical region 8 gene (DGCR8), which is deleted in DiGeorge syndrome (15 ,16 ). In this chapter, we detail the methods used for the biochemical isolation and identification of the Microprocessor complex from human cells. We include a protocol
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