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文献和实验Preparation of Heterogeneous Nuclear Ribonucleoprotein Complexes
class of nuclear pre-mRNA/mRNA-binding proteins, the heterogeneous nuclear ribonucleoproteins (hnRNPs), to form hnRNP complexes (1 ,2 ). The molecular architecture of these complexes must be exceedingly dynamic because each step in the pre-mRNA
Antisense oligonucleotides have been used to study the structure and function of small nuclear ribonucleoprotein (snRNP) complexes and were adapted and modified for the purification of a variety of RNPs. We describe methods for recombinant
Challenges and Opportunities in Fractionation of Recombinant Human Mannan-Binding Lectin
Mannan-binding lectin is an oligomeric protein of the innate immune system. Both natural MBL as well as recombinant protein is a heterogeneous composition of oligomers containing from two up to at least eight structural units. Recombinant
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