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文献和实验Antisense oligonucleotides have been used to study the structure and function of small nuclear ribonucleoprotein (snRNP) complexes and were adapted and modified for the purification of a variety of RNPs. We describe methods for recombinant
Yeast Nuclear Extract (Small Scale)
)containing DTT and protease inhibitors.Depending on protein pellet size,resuspend in 1.5 - 0.4 ml buffer.This can be done with a small dounce homogenizer or a blue pipette tip depending on the amount of protein. Dialyze nuclear extract vs Buffer C + 75 mM
Yeast Nuclear Extract (Small Scale)
tubes and pellet crude nuclei.Spin 13 K rpm for 30 min.in SS34 rotor.Remove supernatant by dumping and discard.Drain pellets. Resuspend crude nuclear pellets with a small spatula in 10 ml Buffer B and transfer to 50 ml screwcap tubes.The prep
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