Recombinant Human ER Lipid Raf

Isolation of Human Brain Endothelial Cells and Characterization of Lipid Raft-Associated Proteins by Mass Spectroscopy

a robust human BECs isolation protocol, standard tissue culture protocols, ECs purity assessment protocols, lipid raft microdomain isolation method, and a mass spectrometry analysis technique to characterize the protein content of membrane microdomains.

Proteomic Study of Caveolae and Rafts Isolated From Human Endothelial Cells

of these subcellular lipid domains has been implicated in a variety of patho-physiological states of the vasculature, and is receiving increased attention. As a result of the membranous composition and abundance of insoluble intrinsic and membrane-associated proteins

Production of Cells with Targeted Integration of Gene Variants of Human ABC Transporter for Stable and Regulated Expression Using the Flp Recombinase

randomly at unpredictable sites in the host’s chromosomal DNA, and the number of integrated recombinant DNAs is not controllable. To overcome this problem, we developed the Flp-In method to integrate one single copy of cDNA encoding the human ABC