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文献和实验Protein Folding Liquid Chromatography
and a new chromatographic cake. A protocol for fast renaturation with simultaneous purification of inclusion body protein of the recombinant human interferon-gamma to obtain purity ≥95% and high specific bioactivity in a single step and in 1 h is introduced.
Introduction of human 14-3-3 proteins
isolated from eight different human tissues shows that 14-3-3 protein mRNAs are expressed in many tissues in the body.In agreement with previous reports,the highest abundance of RNA hybridizing with this cDNA is seen in the brain.----
Strategies to Optimize Protein Expression in E. coli
. 2009a. Recombinant expression and affinity purification of a novel epididymal human sperm‐binding protein, BSPH1. Mol. Hum. Reprod. 15:105‐114. Lefebvre, J., Boileau, G
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