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文献和实验In-Frame Cloning of Synthetic Genes Using PCR Inserts
any sequence or length can be built using the in-frame cloning method. Genes are assembled such that open reading frames are maintained by linking DNA fragments through the use of six basepair blunt-end restriction sites. Each cloning step uses an anchored
Generation of Recombinant Adeno-Associated Virus
open reading frame (ORF) encodes four Rep proteins, Rep78, Rep68, Rep52, and Rep40. The right ORF encodes three Cap proteins, VP1, VP2, and VP3.
top and withdraw a few milliliters of blood. Open the bottle of chromosome medium and place five to ten drops of blood into the medium. Sterile technique must be used because it is possible to cause major contamination during this procedure
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