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文献和实验Assay for Human Rad51-Mediated DNA Displacement Loop Formation
。 Cite as: Cold Spring Harb. Protoc.; 2009; doi:10.1101/pdb.prot5120 Assay for Human Rad51-Mediated DNA Displacement Loop Formation Steven Raynard and Patrick Sung1 Department of Molecular Biophysics and Biochemistry, Yale
【分享】Human press METHODS IN MOLECULAR BIOLOGY
9. Protocols in Human Molecular Genetics. Edited by G. Methaw, 1991 10. Immunochemical Protocols. Edited by Manson, Margaret M., 1992 11. Practical Protein Chromatography Edited by Kenney, Andrew, 1992 12. Pulsed-Field Gel Electrophoresis Protocols
High-Level Expression of a Phage Display-Derived scFv in Pichia pastoris
. Here we describe a large-scale fed-batch fermentation process with an efficient online closed-loop methanol control for the production of the recombinant protein. Purification of A33scFv from clarified culture medium was done using a two-step chromatographic procedure
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