Recombinant Human Signal Recog

信号识别颗粒(signal recognition partical, SRP)

SRP是一种核糖核酸蛋白复合体,沉降系数为11S，含有分子量为72kDa、68kDa、54kDa、19kDa、14kDa及9kDa的6条多肽和一个7S(长约300个核苷酸)的scRNA。 SRP上有三个功能部位: 翻译暂停结构域(P9/P14)、信号肽识别结合位点(P54)、SRP受体蛋白结合位点(P68/P72)。因此, SRP能够识别刚从游离核糖体上合成出来的信号肽，并与之结合，暂时中止新生肽的合成，同时与内质网上的停靠蛋白结合,使核糖体附着到内质网膜上，并进

Introduction of human 14-3-3 proteins

with a 29-kDa protein with partial sequences identical to 14-3-3zeta.In this study,the interaction between GPIb-IX and recombinant 14-3-3zeta is reconstituted.Further,we show that the 14-3-3zeta binding site in GPIb is within a 15 residue sequence at the C

The Use of a Flagellar Export Signal for the Secretion of Recombinant Proteins in Salmonella

flagellin segments containing the export signal combined with a hexahistidine tag can be attached to heterologous proteins (preferentially in the size range of 9–40 kDa) facilitating their secreted expression and easy purification from the medium. Certain