Recombinant Human p53 and DNA-

Identification of p53-Regulated Genes by the Method of Differential Display

DNA damage, is considered to be important for its tumor-suppression function. p53 is a transcription factor that binds to DNA in a sequence-specific manner to activate transcription of target genes. In this chapter, we describe the application

Generation of p53 Target Database Via Integration of Microarray and Global p53 DNA-Binding Site Analysis

sequences in regulatory regions that match the p53 DNA-binding consensus sequence, resulting in the p53 Target Database. This database collects human genes that have at least one p53 DNA-binding sequence in their regulatory region. cDNA microarray

Production of Cells with Targeted Integration of Gene Variants of Human ABC Transporter for Stable and Regulated Expression Using the Flp Recombinase

randomly at unpredictable sites in the host’s chromosomal DNA, and the number of integrated recombinant DNAs is not controllable. To overcome this problem, we developed the Flp-In method to integrate one single copy of cDNA encoding the human ABC