Recombinant Human Tumour Type

A Simplified Method of Screening for Isolation of Recombinant Vaccinia Virus

the wild-type and recombinant viruses at appropriate dilutions are used to infect cell monolayers of human TK− cells, and then overlaid with growth medium containing agarose and 5-BrdU to prevent spreading of recombinant virus plaques and inhibit

Identification of a Mutant Kinase/ATP Analog Pair

, it is necessary to screen ATP analogs for their compatibility with the kinase mutant. This step requires an active form of the wild-type and mutant kinase, either as recombinant activated protein, or as protein that has been immunoprecipitated from transfected

Introduction of human 14-3-3 proteins

whose members have been shown to activate tyrosine and tryptophan hydroxylases,inhibit protein kinase C and possess phospholipase A2 activity in vitro.We have isolated and analyzed a 14-3-3 protein cDNA clone (H14-3-3)from a human fetal brain cDNA library