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文献和实验and p75) (2 ). Signaling through the p55 receptor is primarily associated with responses such as cytotoxicity (2 ,3 ) and cytokine secretion (4 ) whereas the p75 receptor is responsible for lymphoproliferative signals and the activation of T-cells
Introduction of human 14-3-3 proteins
acid sequence of human 14-3-3 protein eta was highly homologous to that of previously reported bovine and rat 14-3-3 proteins with only two amino acid differences.The sequence carries structural features as putative regions responsible for activation
EXAMINING THE FUNCTION OF PROTEINS AND PROTEIN NETWORKS WITH THE YEAST TWO-HYBRID SYSTEM
are expressed from multicopy (2µ) plasmids; the LexA fusion, or bait, is expressed from a plasmid containing the HIS3 marker, and the activation domain fused protein, sometimes called the prey , is expressed from a plasmid containing the TRP1 marker
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