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文献和实验Enzyme-Linked ImmunoSorbent Assay (ELISA) for NGF
A. DAY 1 PROCEDURE• ABSORPTION OF THE POLYCLONAL AND PREIMMUNE SERUMThe Goat Anti-Mouse (GAM) NGF polyclonal antibody recognizes 2.5B NGF and the Goat IgG (GIG) preimmune serum serves as a control for the antibody (Hazleton). The relative signal
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
/) 96‐well plate (8 well removable strips) coated with polyclonal rabbit anti‐PINP antibody (from Competitive ELISA kit; IDS Ltd, http://www.idsplc.com/) Precision
Tips and hints for the storage of antibodies
! Repetitive freeze-thawing of dilute purified IgG is almost certain to lead to substantial losses. Rabbit antisera Antisera are more robust than monoclonals. With anti-microbials added, they may be stored at ℃. Serum does not contain active proteases
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