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文献和实验Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli
The methods described in this article are relative to the use of a positive cloning/screening recombinant system for the generation in Escherichia coli of foreign proteins fused to a highly active bacterial alkaline phosphatase (PhoA) variant
Utilizing Peptide SPOT Arrays to Identify Protein Interactions
Rudiger, S., Germeroth, L., Schneider‐Mergener, J., and Bukau, B. 1997. Substrate specificity of the DnaK chaperone determined by screening cellulose‐bound peptide libraries. EMBO J. 16:1501‐1507.
Transformation of Plasmids/Cosmids into E. Coli 质粒、粘粒转化大肠杆菌
because non- recombinant colonies (bacteria with either uncut vector or re-circularized cut vector) turn a faint blue color on the plate while colonies harboring plasmids containing a cloned insert will remain white (see below for color selectability
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