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文献和实验Yeast transformation using lithium acetate (rapid method)
. Spin 2 min in clinical centrifuge. Resuspend cell pellet in 2 ml TE + 0.1 M lithium acetate. Incubate on tube roller at 30o for 1 hr (can be incubated for up to four hours with no ill effects). Boil high molecular weight salmon sperm DNA
Chromosomal DNA Extraction from Gram-positive Bacteria
extract lysed cells,and ethanol precipitate the DNA with 0.1 vol.3 M sodium acetate,pH 4.8 and 2 vol.95% ethanol. 5.Spool out DNA with a glass rod,wash once with 80% ethanol before drying. Some bacterial species may require a longer incubation
PLATE Transformation "PLATE" is an acronym of the names of ingredients in the transformation solution: PEG, Lithium Acetate, Tris, and EDTA. -Use sterile technique and sterile solutions throughout this method
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