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文献和实验秦奋 为什么HIV-gp41与原核PET28a质粒构建后,行诱导表达,SDS-PAGE无结果,而WB却有结果,这种情况怎么回事?(构建后测序同源性达95%,而且已酶切认证构建成功) 谢谢 zhujoker “SDS-PAGE无结果,而WB却有结果”是什么意思,你表述清楚一点,要不人家会有疑惑的。其实这主要看你是否诱导成功。个人认为还有就是你在原核里面表达,作为包涵体存在形式,你是否将蛋白提出来了呢?
. We have created a platform in which recombinant mucin-type immunoglobulin fusion proteins are used as scaffolds for multivalent expression of O -glycans with diagnostic or therapeutic potential. The methods used to make stable CHO cell lines secreting a mucin-type
Expression of Recombinant Membrane-Type MMPs
” (1 ) there has been much interest in the production of recombinant protein to facilitate both structural and functional analyses. The proteolytic activity of the membrane-type-1 MMP (MT1-MMP) has been studied using recombinant material produced from expression in E. coli (2 ,3 ). These data
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