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文献和实验membrane washes. However, it can be more readily adapted to high-sensitivity chemiluminescent detection with alkaline phosphatase-catalyzed dioxetane substrates. It can also be used with oligonucleotide probes, in this case coupled to horseradish peroxidase
Troubleshooting Tips for Western Immunoblotting
system may need to be used. Inhibition of secondary antibody conjugate: Horseradish peroxidase labeled antibodies should not be used in conjunction with sodium azide or hemoglobin. Biotinylated antibodies should not be used with milk or casein since both contain biotin
Troubleshooting Tips for Western Immunoblotting
: this can occur especially with monoclonal antibodies that were raised against a native protein. In some cases, a non-reducing gel system may need to be used. Inhibition of secondary antibody conjugate: Horseradish peroxidase labeled antibodies
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