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文献和实验Design and Application of a shRNA-Based Gene Replacement Retrovirus
to achieve this much more simply in mammalian cells. A single retrovirus has been constructed, which directs expression of a short hairpin RNA (shRNA) to knockdown expression of the endogenous protein of interest; a cDNA coding for a wild-type or mutant
Assaying Endogenous Phosphatidylinositol-4-Phosphate 5-Kinase (PIP5K) Activities
it. Phosphatidylinositol-4-phosphate 5-kinases (PIP5 K) phosphorylate PtdIns4P on the 5-position and constitute the major pathway for the generation of PtdIns(4,5)P 2 . We will discuss how to suppress the expression of human PIP5 Kβ using RNAi and how to measure
Imaging of Endogenous RNA Using Genetically Encoded Probes
of endogenous mitochondrial RNA in single living cells. Nat. Methods 4:413‐419. Tilsner, J., Linnik, O., Christensen, N.M., Bell, K., Roberts, I.M., Lacomme, C., and Oparka, K.J. 2009
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