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文献和实验The Sheep Erythrocyte T-Dependent Antibody Response (TDAR)
The sheep erythrocyte T-dependent antibody Response (TDAR) evaluates the ability of animals sensitized in vivo to produce primary IgM antibodies to sheep erythrocytes (sRBC). The assay enumerates the number of antigen specific IgM antibody
methods. This process may consist of purification of total IgG or subpopulations (e.g., subclasses) of IgG from antisera/ascitic fluid/culture supernatant or the isolation of a particular antigen-binding fraction of Ig from such fluids
Antigen Design Sera Purification Tech Sheet
control measures (mass spec and/or analytical HPLC). An indirect synthesis of the MAP can eliminate analysis problems. In the indirect method, the peptide is first synthesized, purified then analyzed using mass spec and analytical HPLC. The peptide antigen
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