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文献和实验Antigen Design Sera Purification Tech Sheet
control measures (mass spec and/or analytical HPLC). An indirect synthesis of the MAP can eliminate analysis problems. In the indirect method, the peptide is first synthesized, purified then analyzed using mass spec and analytical HPLC. The peptide antigen
Immunofluorescent Staining of Mouse and Rat Leukocytes
receptors), a three-step protocol may amplify the staining: use purified primary antibody (steps 2-4 of above procedure), biotinylated anti-Ig for the 2nd step (steps 6-7, above), and fluorochrome-conjugated avidin or streptavidin as the 3rd step (repeat
FITC荧光二抗?――EarthOx新型DyLight 488绿色荧光
的价格比常规二抗 只高20%左右,但是Dylight的效价通常是常规荧光二抗的2-4倍左右,因此就最终的性价比,Dylight更胜一筹。举例来说,EarthOx的 FITC标记的山羊抗小鼠IgG二抗(货号为E031210),100μl和500μl报价分别是¥160和¥640;而相应的EarthOx的 Dylight 488标记的山羊抗小鼠IgG二抗(货号为E032210),100μl和500μl报价分别是¥200和¥800,但是在具体使用时,比如免疫荧光实验,其推荐稀释比率分别为1:100
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