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- 2025年07月11日
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文献和实验Anti-DYKDDDDK tag (L5) Affinity Gel
or 0.22µm filter to prepare for L5 purification. Part II. Resin Preparation 1. Thoroughly suspend the resin by gentle inversion until the bottle of Anti- DYKDDDDK-tag (L5) affinity gel is a uniform suspension of gel beads. Transfer
-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
at 1:200 dilution) Cy3-conjugated affinity-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
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