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- CLF311-TR
- 2025年06月29日
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文献和实验Immunofluorescent Staining of Mouse and Rat Leukocytes
receptors), a three-step protocol may amplify the staining: use purified primary antibody (steps 2-4 of above procedure), biotinylated anti-Ig for the 2nd step (steps 6-7, above), and fluorochrome-conjugated avidin or streptavidin as the 3rd step (repeat
Clinical Chemistry and Other Laboratory Tests on Mouse Plasma or Serum
; (3) enzyme‐conjugated antigen is added, unlabeled antigen from samples and the enzyme‐conjugated antigen compete for binding to the capture antibody; (4) substrate is added, and is converted by enzyme to detectable form (e.g., color change into yellow
with different antibodies for simultaneous analysis, you may encounter problems choosing secondary antibodies (fluorochrome conjugated antibodies that bind to the primary antibody). For example, if both primary antibodies are mouse IgG 1, what can you do to label
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