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文献和实验Antigen Design Sera Purification Tech Sheet
will bind to chicken Ig. Care should be taken when eluting the antibody from the column to avoid denaturation of the antibody. Immunoaffinity Purification The most commonly used method to purify antigen-specific antibodies from crude sera
Antigen Design Sera Purification Tech Sheet
to check this before deciding which method to use. For example, Protein G works well with sheep Ig, but Protein A does not. Neither Protein A nor Protein G will bind to chicken Ig. Care should be taken when eluting the antibody from the column to avoid
Studies of the Ubiquitin Proteasome System
polyclonal rabbit anti‐ubiquitin (commercially available or prepared as in protocol 31 ) or monoclonal anti‐ubiquitin in TBST/BSA TBST buffer (see recipe ) [125 I]protein A (MP
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