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文献和实验ELISPOT (Enzyme-linked ImmunoSPOT)
for detection of antigen-specifi c memory effector CD4 and CD8 cell populations in human diseases. J. Immunol. Methods. 285: 223-235. Smith JG, et al. 2001. Development and validation of a gamma interferon ELISPOT assay for quantitation of cellular immune
Introduction of human 14-3-3 proteins
of the tryptic peptides derived from the purified enzyme revealed two amino acid sequences homologous to the 14-3-3 proteins.A polyclonal antibody was raised against purified ST-IV.A 33 kDa protein was co-immunoprecipitated from rat brain extracts with the anti
Strategies to Optimize Protein Expression in E. coli
Tanaka, T., Kubota, M., Samizo, K., Nakajima, Y., Hoshino, M., Kohno, T., and Wakamatsu, E. 1999. One‐step affinity purification of the G protein beta gamma subunits from bovine brain using a histidine‐tagged G protein alpha subunit. Protein Expr
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