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文献和实验Studies of the Ubiquitin Proteasome System
of Unstable E3s in Bacterial Lysates Basic Protocol 25: Expression of E3s in Eukaryotic in Vitro Translation Systems Basic Protocol 26: Expression of Multi‐Subunit E3s Using a Baculovirus Expression System Support Protocol 5: Batch Purification of Multimeric E
Selection of Non-aggregating VH Binders from Synthetic VH Phage-Display Libraries
approaches for developing non-aggregating human VH s with binding specificities have relied on a combination of recombinant DNA technology and phage-display technology. VH gene libraries are constructed synthetically by randomizing the CDRs of a single VH
Recombinant Adeno-Associated Viral Vector Production and Purification
. In general, AAV vectors lead to robust, long-term in vivo transduction in rodents, dogs, and non-human primates. To meet specific research needs, investigators have developed numerous AAV variants by engineering viral capsid and/or genome. Here we outline
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