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文献和实验Conjugating DNA into Mycobacteria
between gram-negative and gram-positive bacteria was first demonstrated using recombinant-shuttle plasmids (1 ). In Mycobacterium, the shuttle-plasmid pMY10 was introduced to Mycobacterium smegmatis from Escherichia coli (2 ), as was the naturally occurring
Medium-Throughput Production of Recombinant Human Proteins: Protein Production in E. coli
In Chapter 4 we described the SGC process for generating multiple constructs of truncated versions of each protein using LIC. In this chapter we provide a step-by-step procedure of our E . coli system for test expressing intracellular
Studies of the Ubiquitin Proteasome System
and Purification of Anti‐Ubiquitin Antibodies Support Protocol 3: Preparation of Ubiquitin Affinity Column Generation of Reagent‐Grade Ubiquitin Activating Enzyme (E1) Basic Protocol 20: Preparation of Wheat E1 in E. coli Basic Protocol 21: Preparation of Mouse E1
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