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文献和实验Activity Assays for Poly-ADP Ribose Polymerase
Poly(ADP-ribose) polymerase (PARP-1) is a nuclear enzyme that has traditionally been thought to require discontinuous or “damaged” DNA (dcDNA) as a coenzyme, a preconception that has limited research mainly to its role in cell pathology, i.e
Purification of Recombinant Poly(ADP-Ribose) Polymerases
The purification of Poly(ADP-ribose) polymerases from overexpressing cells (Sf9 insect cells, Escherichia coli ) has been updated to a fast and reproducible three chromatographic steps protocol. After cell lysis, proteins from the crude
Markers of Poly (ADP-Ribose) Polymerase Activity as Correlates of DNA Damage
Poly (ADP-ribose) polymerase (PARP-1; EC 2.4.2.30) is a predominantly nuclear enzyme long presumed to function in the identification and repair of DNA strand nicks and breaks (for recent reviews see: 1 –3 ). PARP is comprised of a N
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