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文献和实验Assaying Phospholipase A2 Activity
Mammalian cells contain many structurally and functionally diverse phospholipases A2 (PLA2 ) that catalyze the hydrolysis of sn-2 fatty acid from membrane phospholipid. Assays are described for measuring the activity of Group IVA cytosolic
Efficient Immobilization of Phospholipase A2
Immobilized enzymes have been widely used for biomedical and industrial applications. Since phospholipase A2 (PLA2 ; E.C.3.1.1.4) catalyzes the hydrolysis of a wide range of phospholipid aggregates, immobilized PLA2 should also have potential
High-Throughput Assay of Secreted Phospholipases A2 Inhibitors
Attempts to characterize, quantify, and/or modulate the activity of the secreted phospholipase A2 family of enzymes result from the diversity of physiological roles for which these enzymes have been implicated. The 1-palmitoyl-2-(10
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