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文献和实验Recombinant Feline Immunodeficiency Virus Vectors: Preparation and Use
Traditional onco-retroviral vector packaging systems, typified by those based on Moloney murine leukemia virus (MMLV), have many appealing features for gene transfer. MMLV vectors stably integrate their genome into their target cells
, we describe the generation of recombinant coronaviruses and stable cell lines containing coronaviral replicon RNAs. Initially, the vaccinia virus-based reverse genetic system was established for the generation of recombinant human coronavirus 229
for reverse transcription-polymerase chain reactions (RT-PCR) (1 -4 ). Because they only synthesize short cDNA fragments, their use is not widespread. In general, avian myeloblastosis virus (AMV), or moloney murine leukemia virus (M-MLV) reverse
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