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文献和实验Purifi cation of Multiprotein Histone Acetyltransferase Complexes
The reversible acetylation of specific lysine residues on core histones regulates gene transcription in eukaryotes. Since the discovery of GCN5 as the first transcription-regulating histone acetyltransferase (HAT), a variety of HATs
Transcriptional Activation Analysis by the Chloramphenicol Acetyl Transferase (CAT) Enzyme Assay
reporter genes can be used to measure transcription activity, such as chloramphenicol acetyl transferase (CAT), luciferase, (β-galactosidase, and human growth hormone (7 ). To examine the transcription enhancement activity of a putative promoter/enhancer
Purification, Measurement of Concentration, and Functional Complement Assay of Human Ficolins
Ficolins constitute a group of lectins involved in innate immunity. L-Ficolin, H-ficolin, and M-ficolin are present in human serum. The human ficolins differ in carbohydrate-binding specificity, but they have in common the ability
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