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文献和实验Generation of Recombinant Baculovirus DNA in E.coli Using a Baculovirus Shuttle Vector
of the Autographa californica nuclear polyhedrosis virus (AcNPV) are abundantly expressed during the late stages of infection. The recombinant proteins are usually soluble and functionally similar to their authentic counterparts (l –7 ). In the following sections
二磷酸尿甘半乳糖 uridine diphosphate ga-lactose
上,上述的尿苷酰转移反应是使作为营养源供给的半乳糖转变为1-磷酸葡萄糖,并与糖酵解系统或糖互变系统汇合的反应。与此不同,在具有UDP-半乳糖焦磷酸化酶(UDP galactose pyro-phosphorylase,EC 2.7.7.10)的细胞里由于1-磷酸α-D-半乳糖 UTPUDP半乳糖 ppi的反应而形成UDP-半乳糖。例如,人的肝脏在胎儿或幼儿期几乎检测不出此酶的活性,但其活性随着年龄的增长而增加,从而进行半乳糖代谢。半乳糖血症(galactosemia)虽然有尿苷酰转移酶的遗传性缺失
Expression of Recombinant Alkaline Phosphatase Conjugates in Escherichia coli
The methods described in this article are relative to the use of a positive cloning/screening recombinant system for the generation in Escherichia coli of foreign proteins fused to a highly active bacterial alkaline phosphatase (PhoA) variant
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