Recombinant Human Seryl-tRNA S

Methods to Assay Inhibitors of tRNA Synthetase Activity

could be a potential antibacterial agent. Only one aa-RS inhibitor, the Ile-RS inhibitor mupirocin, is currently marketed as an antibacterial agent. We focused on phenylalanyl (Phe)-tRNA synthetase (Phe-RS), but the described methods are not restricted to Phe-RS

合成酶 synthetase

　　酶分类的主要类别之一。为催化与 ATP等三磷酸核苷的分解伴有形成新化学键可逆反应的酶之总称。催化具有有机化学意义的合成反应，分为两个分子相加的裂解酶和移换反应的移换酶两类。所以还是将不伴随腺苷三磷酸的分解而催化合成反应的酶称为合成酶（ Synthase）较为适宜。合成酶催化的反应含 ATP等基质，反应复杂， ATP分解为 ADP与正磷酸或 AMP与焦磷酸。根据生成键的种类而更分为：（ 1） C-O键，例如氨酰 tRNA合成酶；（ 2） C-S键，例如乙酰 CoA合成

Directed Evolution of the Substrate Specificities of a Site-Specific Recombinase and an Aminoacyl-tRNA Synthetase Using Fluorescence-Activated Cell So

A variety of in vivo selection and screening methods have been developed for the directed evolution of protein function. Typically, in vivo selection strategies involve the identification of new binding or catalytic functions based