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文献和实验Studies of the Ubiquitin Proteasome System
or other proteasome inhibitor (e.g., lactacystin; Calbiochem or Biomol) Phosphate‐buffered saline (PBS; see recipe ) Nondenaturing Triton X‐100 lysis buffer (see recipe ) containing 1×
to establish the rules governing structure/activity relationships of the human 20S proteasome. This chapter describes the use of a combination of proteomic approaches to characterize the human 20S proteasome subunit isoforms separated by 2D gel electrophoresis
Purification of Recombinant Calpastatin Expressed in Escherichia coli
Calpastatin is an endogenous inhibitor protein specific for calpain (1 ,2 ). Other calpain-inhibiting reagents such as EDTA, E-64, leupeptin, and calpain inhibitor I (N -acetyl-L-leucyl-L-leucyl-L-norleucinal), are frequently used
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