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文献和实验Culturing BG01V Human Embryonic Stem Cells with Mouse Embryonic Fibroblast (MEF)-Conditioned Media
If culturing in the absence of a feeder cell layer is desired, human embryonic stem (hES) cells can be maintained using Mouse or Human-Conditioned Media (Catalog # AR005 , AR007 ). The protocol below has been used with the BG01V line
DNA Transfection of Mammalian Cells Using Polybrene
and recombinant DNA sequences for some cell lines, e.g., mouse L-cells, but less well with other cell types such as Chinese hamster ovary (CHO) cells (2 –4 ). Since the ability to rescue mutant phenotypes by exogenous DNA provides a means to identify gene
Identifying Functional Adenovirus-Host Interactions Using Tandem Mass Spectrometry
knob, which is the moiety within the Ad capsid responsible for high-affinity attachment of virus to cellular receptors. We used recombinant fiber knob domains from members of group C and B Ads to purify virus interacting proteins from cell membrane
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