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文献和实验1:膜蛋白应用实例 蛋白表达系统纯度产率 (所有回收蛋白)采取的纯化步骤hCB2 G蛋白偶联受体大肠杆菌90%4.5mg/5L 培养物Rho1D4 IAC+SEChVN1R1 G蛋白偶联受体可诱导的HEK293S细胞系(哺乳动物)90%1mg/1g细胞Rho1D4 IAC+SECFPR3 G蛋白偶联受体可诱导的HEK293S细胞系(哺乳动物)90%2mg/6g细胞Rho1D4 IAC+SECTAAR5 G蛋白偶联受体可诱导的HEK293S细胞系(哺乳动物)90
. In particular, the use of genetically engineered mice (e.g., mice that have specific genes deleted or overexpressed) allows one to investigate relevant key factors for neovascularization that can be related to human subjects (3 -6 ). Accordingly, we developed
2 is commonly overexpressed in human breast cancers and is a marker of poor prognosis. Expression vectors for short hairpin RNAs (shRNA) that target CpG islands within the ERBB2 promoter decrease the amount of mRNA and induce promoter methylation. Consequently
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