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文献和实验Notes on Making Rat x Y3 Monoclonal Antibody Producing Hybridomas
of one rat spleen (approx. 10**8 cells) together with 6 x 10**7 Y3/Ag1.2.3 per fusion (the other half of the spleen cells can be frozen as a backup in 90% FCS +10% DMS0). c) Try and keep all cells, PEG and medium components at 37 deg. C
Immunofluorescent Staining of Mouse and Rat Leukocytes
16 (Mouse BD Fc Block™; Cat No. 553141/553142) or anti-rat CD32 (Rat BD Fc Block™; Cat. No. 550270/550271) is recommended. BD Fc Block™ can be added to cells (~0.25 µg per million cells, 3 - 5 min, 4°C) and need not be washed out prior to addition of primary
BLOCK-iT Fluorescent Oligo as RNAi Transfection Control
The BLOCK-iT™ Fluorescent Oligo is light sensitive. Store the stock solution at -20°C, protected from light. The stock solution is stable for at least 6 months if stored properly. 2) When using, thaw the stock solution on ice or at room
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